CLOCK Rabbit mAb, Unconjugated, Monoclonal

Product Overview

• Article Name: CLOCK Rabbit mAb, Unconjugated, Monoclonal
• Biozol Catalog Number: ABB-A7265
• Supplier Catalog Number: A7265
• Alternative Catalog Number: ABB-A7265-20UL,ABB-A7265-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: KAT13D, bHLHe8, CLOCK

Product Description

The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants.

Product Properties

• Clonality: Monoclonal
• Molecular Weight: 95kDa
• NCBI: 9575
• UniProt: O15516
• Purity: Affinity purification
• Target: CLOCK

Application Notes

• Application Dilute: WB,1:1000 - 1:6000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Signal Transduction,Endocrine Metabolism,Lipid Metabolism,Neuroscience,Cardiovascular,Blood,Heart. Shipping: Ice Bag

Images

Western blot analysis of various lysates using CLOCK Rabbit mAb (A7265) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.