NME1/NM23A Rabbit mAb, Unconjugated

Product Overview

• Article Name: NME1/NM23A Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-A8802
• Supplier Catalog Number: A8802
• Alternative Catalog Number: ABB-A8802-20UL, ABB-A8802-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: NB, AWD, NBS, GAAD, NDKA, NM23, NDPKA, NDPK-A, NM23-H1, NME1/NM23A

Product Description

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of A (encoded by this gene) and B (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

Product Properties

• Molecular Weight: 17 kDa
• NCBI: 4830
• UniProt: P15531
• Purity: Affinity purification
• Target: NME1

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Cancer,Tumor suppressors,Cell Biology Developmental Biology,Apoptosis,Neuroscience,Neurodegenerative Diseases Markers,Other Neurological disorders.

Images

Western blot analysis of various lysates using NME1/NM23A Rabbit mAb (A8802) at 1:1000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20 s.