Thioredoxin reductase 2 (TXNRD2 ) Rabbit mAb, Unconjugated

Product Overview

• Article Name: Thioredoxin reductase 2 (TXNRD2 ) Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-A8884
• Supplier Catalog Number: A8884
• Alternative Catalog Number: ABB-A8884-20UL, ABB-A8884-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: TR, TR3, SELZ, GCCD5, TRXR2, TR-BETA, Thioredoxin reductase 2 (TXNRD2 )

Product Description

The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes a mitochondrial form important for scavenging reactive oxygen species in mitochondria. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3 UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms, including a few localized in the cytosol and some lacking the C-terminal Sec residue, have been found for this gene.

Product Properties

• Molecular Weight: 57kDa
• NCBI: 10587
• UniProt: Q9NNW7
• Purity: Affinity purification
• Target: TXNRD2

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Cell Biology Developmental Biology,Endocrine Metabolism.

Images

Western blot analysis of various lysates using Thioredoxin reductase 2 (TXNRD2 ) Rabbit mAb (A8884) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10 s.