APPL1 Rabbit mAb, Unconjugated

Product Overview

• Article Name: APPL1 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-A8932
• Supplier Catalog Number: A8932
• Alternative Catalog Number: ABB-A8932-100UL, ABB-A8932-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: APPL, MODY14, DIP13alpha, APPL1

Product Description

The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus.

Product Properties

• Molecular Weight: 80 kDa
• NCBI: 26060
• UniProt: Q9UKG1
• Purity: Affinity purification
• Target: APPL1

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Signal Transduction,Cell Biology Developmental Biology,Apoptosis,Neuroscience.

Images

Western blot analysis of various lysates using APPL1 Rabbit mAb (A8932) at 1:1000 dilution incubated overnight at 4C
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30 s.