Enables DNA-binding transcription activator activity, RNA polymerase II-specific, RNA polymerase II cis-regulatory region sequence-specific DNA binding activity, and transcription regulator inhibitor activity. Involved in brain development, negative regulation of DNA binding activity, and regulation of transcription by RNA polymerase II. Located in fibrillar center and nucleoplasm.
Western blot analysis of various lysates using NFIB / NF1B2 Rabbit mAb (A9125) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
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