RFK Rabbit pAb, Unconjugated

Product Overview

• Article Name: RFK Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-A9141
• Supplier Catalog Number: A9141
• Alternative Catalog Number: ABB-A9141-100UL,ABB-A9141-20UL,ABB-A9141-1000UL,ABB-A9141-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: RIFK, RFK

Product Description

Riboflavin kinase (RFK, EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 18kDa
• NCBI: 55312
• UniProt: Q969G6
• Purity: Affinity purification
• Sequence: MRHLPYFCRGQVVRGFGRGSKQLGIPTANFPEQVVDNLPADISTGIYYGWASVGSGDVHKMVVSIGWNPYYKNTKKSMETHIMHTFKEDFYGEILNVAIVGYLRPEKNFDSLESLISAIQGDIEEAKKRLELPEHLKIKEDNFFQVSKSKIMNGH
• Target: RFK
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse, ResearchArea: Signal Transduction,Endocrine Metabolism.

Images

Western blot analysis of various lysates using RFK Rabbit pAb (A9141) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.