14-3-3 alpha/beta Rabbit mAb, Unconjugated

Product Overview

• Article Name: 14-3-3 alpha/beta Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-A9151
• Supplier Catalog Number: A9151
• Alternative Catalog Number: ABB-A9151-20UL, ABB-A9151-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: HS1, GW128, YWHAA, KCIP-1, HEL-S-1, 14-3-3 alpha/beta

Product Description

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.

Product Properties

• Molecular Weight: 28kDa
• NCBI: 7529
• UniProt: P31946
• Purity: Affinity purification
• Target: YWHAB

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Signal Transduction,G protein signaling,Kinase,PI3K-Akt Signaling Pathway,MAPK-Erk Signaling Pathway,MAPK-JNK Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Mitochondrial Control of Apoptosis,Cell Cycle,Cell Cycle Control-G2 M DNA Damage Checkpoint,Immunology Inflammation,Neuroscience, Cell Type Marker,Stem Cells,Neuron marker,Synapse marker.

Images

Western blot analysis of various lysates using 14-3-3 alpha/beta Rabbit mAb (A9151) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.