14-3-3-beta/zeta Rabbit mAb, Unconjugated

Catalog Number: ABB-A9156
Article Name: 14-3-3-beta/zeta Rabbit mAb, Unconjugated
Biozol Catalog Number: ABB-A9156
Supplier Catalog Number: A9156
Alternative Catalog Number: ABB-A9156-20UL, ABB-A9156-100UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: GW128, HEL-S-1, HS1, KCIP-1, YWHAA, 14-3-3-beta/zeta
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
Molecular Weight: 28kDa
NCBI: 7529
UniProt: P31946
Purity: Affinity purification
Target: YWHAB/YWHAZ
Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Neuroscience,Neurology process,Neural Signal Transduction,Signal Transduction,Adapters,Cytoplasmic,Stem Cells,Signaling Pathways.
Western blot analysis of various lysates using 14-3-3-beta/zeta Rabbit mAb (A9156) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.