Cytokeratin 12 (KRT12) Rabbit pAb, Unconjugated

Product Overview

• Article Name: Cytokeratin 12 (KRT12) Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-A9642
• Supplier Catalog Number: A9642
• Alternative Catalog Number: ABB-A9642-100UL, ABB-A9642-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: K12, MECD1, Cytokeratin 12 (KRT12)

Product Description

KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.

Product Properties

• Molecular Weight: 54kDa
• NCBI: 3859
• UniProt: Q99456
• Purity: Affinity purification
• Target: KRT12

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Signal Transduction,Cell Biology Developmental Biology,Cytoskeleton,Intermediate Filaments,Neuroscience, Cell Type Marker.

Images

Western blot analysis of various lysates using Cytokeratin 12 (KRT12) Rabbit pAb (A9642) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.