MT-CYB Rabbit pAb, Unconjugated

Catalog Number: ABB-A9762
Article Name: MT-CYB Rabbit pAb, Unconjugated
Biozol Catalog Number: ABB-A9762
Supplier Catalog Number: A9762
Alternative Catalog Number: ABB-A9762-100UL, ABB-A9762-20UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Mouse
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: CYTB, MT-CYB
Predicted to enable metal ion binding activity and ubiquinol-cytochrome-c reductase activity. Predicted to be involved in several processes, including electron transport coupled proton transport, mitochondrial electron transport, ubiquinol to cytochrome c, and response to glucagon. Located in membrane and mitochondrion. Is integral component of mitochondrial inner membrane. Human ortholog(s) of this gene implicated in ovarian carcinoma and urinary bladder cancer. Orthologous to human MT-CYB (mitochondrially encoded cytochrome b).
Molecular Weight: 43kDa
NCBI: 17711
UniProt: P00158
Purity: Affinity purification
Target: mt-Cytb
Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Signal Transduction,Endocrine & Metabolism,Mitochondrial metabolism,Cytochromes,Neuroscience,Neurodegenerative Diseases.
Western blot analysis of lysates from Mouse heart, using MT-CYB Rabbit pAb (A9762) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.