CDC34 Rabbit mAb, Unconjugated

Catalog Number: ABB-A9772
Article Name: CDC34 Rabbit mAb, Unconjugated
Biozol Catalog Number: ABB-A9772
Supplier Catalog Number: A9772
Alternative Catalog Number: ABB-A9772-100UL, ABB-A9772-20UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: UBC3, UBCH3, UBE2R1, E2-CDC34, CDC34
The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication.
Molecular Weight: 27 kDa
NCBI: 997
UniProt: P49427
Purity: Affinity purification
Target: CDC34
Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Cell Biology Developmental Biology,Ubiquitin,Ubiquitin-Proteasome Signaling Pathway.
Western blot analysis of various lysates using CDC34 Rabbit mAb (A9772) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.