HCRT Rabbit pAb, Unconjugated

Product Overview

• Article Name: HCRT Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-A9821
• Supplier Catalog Number: A9821
• Alternative Catalog Number: ABB-A9821-100UL,ABB-A9821-20UL,ABB-A9821-1000UL,ABB-A9821-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: OX, PPOX, NRCLP1, HCRT

Product Description

This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 13kDa
• NCBI: 3060
• UniProt: O43612
• Purity: Affinity purification
• Sequence: QPLPDCCRQKTCSCRLYELLHGAGNHAAGILTL
• Target: HCRT
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Cancer,Endocrine Metabolism,Endocrine and metabolic diseases,Obesity,Neuroscience.

Images

Western blot analysis of various lysates using HCRT Rabbit pAb (A9821) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.