CAPN10 Rabbit pAb, Unconjugated

Product Overview

• Article Name: CAPN10 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-A9865
• Supplier Catalog Number: A9865
• Alternative Catalog Number: ABB-A9865-20UL,ABB-A9865-100UL,ABB-A9865-1000UL,ABB-A9865-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: CANP10, NIDDM1, CAPN10

Product Description

Calpains represent a ubiquitous, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation, domain II, the protease domain, domain III, a linker domain of unknown function, and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM), and is located within the NIDDM1 region. Multiple alternative transcript variants have been described for this gene.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 75kDa
• NCBI: 11132
• UniProt: Q9HC96
• Purity: Affinity purification
• Sequence: MRAGRGATPARELFRDAAFPAADSSLFCDLSTPLAQFREDITWRRPQEICATPRLFPDDPREGQVKQGLLGDCWFLCACAALQKSRHLLDQVIPPGQPSWADQEYRGSFTCRIWQFGRWVEVTTDDRLPCLAGRLCFSRCQREDVFWLPLLEKVYAKVHGSYEHLWAGQVADALVDLTGGLAERWNLKGVAGSGGQQDRPGRWEHRTCRQLLHLKDQCLISCCVLSPRAG
• Target: CAPN10
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:1000 - 1:4000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Signal Transduction,Cell Biology Developmental Biology,Ubiquitin,Endocrine Metabolism,Endocrine and metabolic diseases,Neuroscience,Calcium Signaling.

Images

Western blot analysis of various lysates using CAPN10 Rabbit pAb (A9865) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.