Agarose Beads-conjugated Mouse anti V5-Tag mAb, Unconjugated
Biozol Catalog Number:
ABB-AE029
Supplier Catalog Number:
AE029
Alternative Catalog Number:
ABB-AE029-500UL
Manufacturer:
ABclonal
Host:
Mouse
Category:
Antikörper
Species Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:
Unconjugated
Alternative Names:
V5,V5 tag,V5-tag
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
Concentration:
IP
Purity:
Affinity purification
Target:
V5
Application Dilute:
IP,20-40ul antibody for 200ug-400ug extracts of whole cells
Application Notes:
Cross-Reactivity: Species independent
Immunoprecipitation of GFP-V5 from 300 µg extracts of 293F cells transfected with a GFP expression vector containing a single N-terminal V5-Tag was performed using 20 µl of Agarose Beads-conjugated Mouse anti V5-Tag mAb (AE029).Affinity Gel-conjugated Mouse anti DDDDK-Tag mAb was used to precipitate the Control IgG sample. IP samples were eluted with 1* Laemmli Buffer. The Input lane represents 10% of the total input.Western blot analysis of immunoprecipitates was conducted usingRabbit anti V5-Tag mAb (AE101) at a dilution of 1:10000.
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