Affinity Gel-conjugated Mouse anti HA-Tag mAb {Anti-HA Affinity Gel}
Biozol Catalog Number:
ABB-AE059
Supplier Catalog Number:
AE059
Alternative Catalog Number:
ABB-AE059-500UL, ABB-AE059-1000UL
Manufacturer:
ABclonal
Host:
Mouse
Category:
Antikörper
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Alternative Names:
HA,HA tag,HA-tag
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
Concentration:
IP
Purity:
Affinity purification
Target:
HA
Application Dilute:
IP,20µl-40µl Affinity Gel for 100µg-300µg extracts of whole cells|Binding Cap,1.5 mg HA protein/mL
Application Notes:
Cross-Reactivity: Human,Species independent
Immunoprecipitation of SCRN1-HA from 300 µg extracts of 293T cells transfected with a SCRN1 expression vector containing a single N-terminal HA-Tag was performed using 30 µl of Affinity Gel-conjugated Mouse anti HA-Tag mAb {Anti-HA Affinity Gel} (AE059). Affinity Gel-conjugated Mouse Control IgG pAb was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10 % of the total input.Western blot analysis of immunoprecipitates was conducted using Mouse anti HA-Tag mAb (AE065) at a dilution of 1:2000.
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