Affinity Gel-conjugated Rabbit anti GFP-Tag mAb {Anti-GFP Affinity Gel}
Biozol Catalog Number:
ABB-AE119
Supplier Catalog Number:
AE119
Alternative Catalog Number:
ABB-AE119-1000UL, ABB-AE119-500UL
Manufacturer:
ABclonal
Host:
Rabbit
Category:
Antikörper
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Alternative Names:
GFP,GFP tag,GFP-tag
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression.
Concentration:
IP
Purity:
Affinity purification
Target:
GFP
Application Dilute:
IP,20µl-40µl Affinity Gel for 100µg-300µg extracts of whole cells
Application Notes:
Cross-Reactivity: Species independent
Immunoprecipitation of GFP-Tag in 150 µg extracts from 293F cells transfected with GFP-Tag using 20 µl Affinity Gel-conjugated Rabbit anti GFP-Tag mAb {Anti-GFP Affinity Gel} (AE119). Western blot analysis was performed using HRP-conjugated Mouse anti GFP-Tag mAb (AE030) at 1:5000 dilution.
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