Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:
Magnetic Beads
Alternative Names:
GST,GST tag,GST-Tag
Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. The GST family consists of three superfamilies: the cytosolic, mitochondrial, and microsomal-also known as MAPEG-proteins. Members of the GST superfamily are extremely diverse in amino acid sequence, and a large fraction of the sequences deposited in public databases are of unknown function. The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions.A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 KDa) in size, which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. However, many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.
Concentration:
IP
Purity:
Affinity purification
Target:
GST-Tag
Application Dilute:
IP,20µl-40µl Magnetic Beads for 100µg-300µg extracts of whole cells
Application Notes:
Cross-Reactivity: Species independent
Immunoprecipitation of GST-Tag from 300 µg extracts of 293F cells transfected with a MYOD1 expression vector containing a single N-terminal GST-Tag was performed using 20 µl of Magnetic Beads-conjugated Rabbit anti GST-Tag mAb (AE122). Magnetic Beads-conjugated Rabbit IgG isotype control(AC047) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10 % of the total input. Western blot analysis of immunoprecipitates was conducted using Rabbit anti GST-Tag mAb (AE077) at a dilution of 1:2000.
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