Magnetic Beads-conjugated Mouse anti GFP-Tag mAb, IgG1
Biozol Catalog Number:
ABB-AE168
Supplier Catalog Number:
AE168
Alternative Catalog Number:
ABB-AE168-500UL
Manufacturer:
ABclonal
Host:
Mouse
Category:
Antikörper
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation:
Magnetic Beads
Alternative Names:
GFP,GFP tag,GFP-tag
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. It has been used in modified forms to make biosensors, and many animals have been created that express GFP, which demonstrates a proof of concept that a gene can be expressed throughout a given organism, in selected organs, or in cells of interest. GFP can be introduced into animals or other species through transgenic techniques, and maintained in their genome and that of their offspring. To date, GFP has been expressed in many species, including bacteria, yeasts, fungi, fish and mammals, including in human cells.
Concentration:
IP
Molecular Weight:
27kDa
Isotype:
IgG1
Purity:
Affinity purification
Target:
GFP
Application Dilute:
IP,30ul antibody (bead slurry) for 200µg-400µg extracts of whole cells
Application Notes:
Cross-Reactivity: Species independent
Immunoprecipitation analysis of 300ug extracts of 293T-SERPINB1-Flag-GFP-N cells using 30ul Magnetic Beads-conjugated Mouse anti GFP-Tag mAb (AE168). Western blot was performed from the immunoprecipitate using Magnetic Beads-conjugated Mouse anti GFP-Tag mAb (AE168) at a dilition of 1:2000.
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