Phospho-eIF4EBP1-S65 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-eIF4EBP1-S65 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0032
• Supplier Catalog Number: AP0032
• Alternative Catalog Number: ABB-AP0032-20UL,ABB-AP0032-500UL,ABB-AP0032-1000UL,ABB-AP0032-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: BP-1, 4EBP1, 4E-BP1, PHAS-I, Phospho-eIF4EBP1-S65

Product Description

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5 end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 13kDa
• NCBI: 1978
• UniProt: Q13541
• Purity: Affinity purification
• Sequence: RNSPV
• Target: EIF4EBP1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Signal Transduction,PI3K-Akt Signaling Pathway,mTOR Signaling Pathway,Endocrine Metabolism,AMPK Signaling Pathway,Insulin Receptor Signaling Pathway,Endocrine and metabolic diseases,Diabetes,Obesity,Cardiovascular,Angiogenesis.

Images

Western blot analysis of lysates from 293T cells usingPhospho-eIF4EBP1-S65 Rabbit pAb (AP0032) at1:700 dilution. 293T cells were treated by lambdaPP mixed solution (1ul) at 37°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:60s.