Phospho-ERK3-S189 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-ERK3-S189 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0051
• Supplier Catalog Number: AP0051
• Alternative Catalog Number: ABB-AP0051-100UL, ABB-AP0051-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: ERK3, PRKM6, p97MAPK, HsT17250, Phospho-ERK3-S189

Product Description

The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.

Product Properties

• Molecular Weight: 83kDa
• NCBI: 5597
• UniProt: Q16659
• Purity: Affinity purification
• Target: MAPK6

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Signal Transduction,G protein signaling,G-Protein-Coupled Receptors Signaling to MAPK Erk,Kinase,Serine threonine kinases,mTOR Signaling Pathway,ErbB-HER Signaling Pathway,Cell Biology Developmental Biology,Microtubules,Immunology Inflammation,Jak-Stat-IL-6 Receptor Signaling Pathway.

Images

Western blot analysis of lysates from 293 cells, using Phospho-ERK3-S189 Rabbit pAb (AP0051) at 1:1000 dilution. 293 cells were treated with PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.