Phospho-STAT1-Y701 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-STAT1-Y701 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP0054
• Supplier Catalog Number: AP0054
• Alternative Catalog Number: ABB-AP0054-500UL,ABB-AP0054-20UL,ABB-AP0054-100UL,ABB-AP0054-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91, Phospho-STAT1-Y701

Product Description

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. The protein encoded by this gene can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. The protein plays an important role in immune responses to viral, fungal and mycobacterial pathogens. Mutations in this gene are associated with Immunodeficiency 31B, 31A, and 31C.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC0049]
• Molecular Weight: 87 kDa
• NCBI: 6772
• UniProt: P42224
• Purity: Affinity purification
• Sequence: TGYIK
• Target: STAT1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Protein phosphorylation,Cancer,Signal Transduction,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Inhibition of Apoptosis,Immunology Inflammation,Jak-Stat-IL-6 Receptor Signaling Pathway.

Images

Western blot analysis of various lysates using Phospho-STAT1-Y701 Rabbit mAb (AP0054) at 1:1000 dilutionincubated overnight at 4°C. HeLa cells, NIH/3T3 cells and PC-12 cells were treated with TNF-alpha (100 ng/mL) at 37°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.