Phospho-MEK1-S217 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-MEK1-S217 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0062
• Supplier Catalog Number: AP0062
• Alternative Catalog Number: ABB-AP0062-100UL,ABB-AP0062-20UL,ABB-AP0062-500UL,ABB-AP0062-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: MEL, CFC3, MEK1, MKK1, MAPKK1, PRKMK1, Phospho-MEK1-S217

Product Description

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 43kDa
• NCBI: 5604
• UniProt: Q02750
• Purity: Affinity purification
• Sequence: IDSMA
• Target: MAP2K1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation,Signal Transduction,G protein signaling,G-Protein-Coupled Receptors Signaling to MAPK Erk,Kinase,Tyrosine kinases,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Cytoskeleton,Actins,ESC Pluripotency and Differentiation,Endocrine Metabolism,Insulin Receptor Signaling Pathway,Warburg Effect,Immunology Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,Jak-Stat-IL-6 Receptor Signaling Pathway,Neuroscience,Neurodegenerative Diseases,Cardiovascular,Angiogenesis.

Images

Western blot analysis of lysates from HeLa cells, using Phospho-MEK1-S217 Rabbit pAb (A12687). HeLa cells were treated with PMA/TPA (200 nM) at 37°C for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.