Phospho-MEK1-T286 Rabbit pAb, Unconjugated
Catalog Number:
ABB-AP0065
- Images (1)
| Article Name: | Phospho-MEK1-T286 Rabbit pAb, Unconjugated |
| Biozol Catalog Number: | ABB-AP0065 |
| Supplier Catalog Number: | AP0065 |
| Alternative Catalog Number: | ABB-AP0065-1000UL,ABB-AP0065-100UL,ABB-AP0065-20UL,ABB-AP0065-500UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | MEL, CFC3, MEK1, MKK1, MAPKK1, PRKMK1, Phospho-MEK1-T286 |
| The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. |
| Application Dilute: | WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation,Signal Transduction,G protein signaling,G-Protein-Coupled Receptors Signaling to MAPK Erk,Kinase,Tyrosine kinases,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Cytoskeleton,Actins,ESC Pluripotency and Differentiation,Endocrine Metabolism,Insulin Receptor Signaling Pathway,Warburg Effect,Immunology Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,Jak-Stat-IL-6 Receptor Signaling Pathway,Neuroscience,Neurodegenerative Diseases,Cardiovascular,Angiogenesis. |
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Western blot analysis of various lysates using Phospho-MEK1-T286 Rabbit pAb (AP0065) at 1:2000 dilution or MEK1 antibody (A12687). HeLa cells were treated with nocodazole (50 ng/mL) at 37°C for 20 hours or treated with ATP(5 mM) at 30°C for 1 hour. NIH/3T3 cells were treated with Nocodazole (50 ng/mL) at 37°C for 20 hours or treated with ATP(5 mM) at 30°C for 1 hour. PC-12 cells were treated with Nocodazole (1 µg/mL) at 37°C for 16 hours or treated with ATP(5 mM) at 30°C for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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