Phospho-Rac1/2/3-S71 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-Rac1/2/3-S71 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0086
• Supplier Catalog Number: AP0086
• Alternative Catalog Number: ABB-AP0086-500UL,ABB-AP0086-20UL,ABB-AP0086-100UL,ABB-AP0086-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: MIG5, MRD48, Rac-1, TC-25, p21-Rac1, Phospho-Rac1/2/3-S71

Product Description

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 21kDa
• NCBI: 5879
• UniProt: P63000
• Purity: Affinity purification
• Sequence: RPLSYP
• Target: RAC1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Rat, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,G protein signaling,Small G proteins,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,MAPK-JNK Signaling Pathway,Cell Biology Developmental Biology,Cytoskeleton,Actins,Wnt -Catenin Signaling Pathway,Immunology Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,Toll-like Receptor Signaling Pathway,Cell Intrinsic Innate Immunity Signaling Pathway,TLR Signaling,Neuroscience,Neurodegenerative Diseases.

Images

Western blot analysis of lysates from A-431 cells, using Phospho-Rac1/2/3-S71 Rabbit pAb (AP0086) at 1:1000 dilution. A-431 cells were treated with EGF (25 µg/mL) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.