Phospho-STAT5A-Y694 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-STAT5A-Y694 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0138
• Supplier Catalog Number: AP0138
• Alternative Catalog Number: ABB-AP0138-20UL,ABB-AP0138-1000UL,ABB-AP0138-100UL,ABB-AP0138-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: MGF, STAT5, Phospho-STAT5A-Y694

Product Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 91kDa
• NCBI: 6776
• UniProt: P42229
• Purity: Affinity purification
• Sequence: DGYVK
• Target: STAT5A
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Protein phosphorylation,Signal Transduction,ErbB-HER Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Immunology Inflammation,Jak-Stat-IL-6 Receptor Signaling Pathway,Stem Cells.

Images

Western blot analysis of lysates from TF-1 cells, using Phospho-STAT5A-Y694 Rabbit pAb (AP0138) at 1:1000 dilution. TF-1 cells were treated with G-CSF (25 ng/ml) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.