Phospho-VAV1-Y174 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-VAV1-Y174 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0180
• Supplier Catalog Number: AP0180
• Alternative Catalog Number: ABB-AP0180-20UL, ABB-AP0180-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: VAV, Phospho-VAV1-Y174

Product Description

This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Product Properties

• Concentration: WB
• Molecular Weight: 98kDa
• NCBI: 7409
• UniProt: P15498
• Purity: Affinity purification
• Target: VAV1

Application Notes

• Application Dilute: WB,1:500 - 1:2000
• Application Notes: Cross-Reactivity: Human,Rat, ResearchArea: Epigenetics Nuclear Signaling,Cancer,Signal Transduction,G protein signaling,Small G proteins,ErbB-HER Signaling Pathway,Cell Biology Developmental Biology,Cell Cycle,Cell differentiation,Cytoskeleton,Actins,Immunology Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway.

Images

Western blot analysis of lysates from Jurkat cells, using Phospho-VAV1-Y174 Rabbit pAb (AP0180).
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.