Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0209
• Supplier Catalog Number: AP0209
• Alternative Catalog Number: ABB-AP0209-20UL,ABB-AP0209-100UL,ABB-AP0209-500UL,ABB-AP0209-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: MEK1/MEK2, Phospho-MAP2K1-S217/MAP2K2-S221

Product Description

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 40kDa/43kDa/44kDa
• NCBI: 5604
• UniProt: Q02750
• Purity: Affinity purification
• Sequence: SMANS
• Target: MAP2K1/MAP2K2
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:2000 - 1:7000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation.

Images

Western blot analysis of lysates from HeLa cells usingPhospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb (AP0209) at1:6000 dilution. HeLa cells were treated with PMA (200 nM) at 37°C for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time:90s.