Phospho-PTK2B-Y402 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-PTK2B-Y402 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0214
• Supplier Catalog Number: AP0214
• Alternative Catalog Number: ABB-AP0214-20UL, ABB-AP0214-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: PKB, PTK, CAKB, FAK2, PYK2, CADTK, FADK2, RAFTK, Phospho-PTK2B-Y402

Product Description

This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene.

Product Properties

• Molecular Weight: 116kDa
• NCBI: 2185
• UniProt: Q14289
• Purity: Affinity purification
• Target: PTK2B

Application Notes

• Application Dilute: WB,1:500 - 1:1000|IF/ICC,1:100 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,G protein signaling,G-Protein-Coupled Receptors Signaling to MAPK Erk,Kinase,Tyrosine kinases,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Endocrine Metabolism,Immunology Inflammation,B Cell Receptor Signaling Pathway.

Images

Western blot analysis of lysates from Jurkat cells, using Phospho-PTK2B-Y402 Rabbit pAb (AP0214) at 1:1000 dilution. Jurkat cells were treated with 10% FBS at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.