Phospho-Camk2-T286 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-Camk2-T286 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0255
• Supplier Catalog Number: AP0255
• Alternative Catalog Number: ABB-AP0255-500UL,ABB-AP0255-1000UL,ABB-AP0255-20UL,ABB-AP0255-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: CAMKA, MRD53, MRT63, CaMKIIalpha, CaMKIINalpha, CAM2, CAMK2, CAMKB, MRD54, CaMKIIbeta, CAMKD, CAMK, CAMKG, MRD59, CAMK-II, Phospho-Camk2-T286

Product Description

The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 54kDa
• NCBI: 816
• UniProt: Q13554
• Purity: Affinity purification
• Sequence: QETVD
• Target: CAMK2
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:2000 - 1:4000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,G protein signaling,G-Protein-Coupled Receptors Signaling to MAPK Erk,Kinase,Serine threonine kinases,ErbB-HER Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Mitochondrial Control of Apoptosis,Microtubules,Endocrine Metabolism,Carbohydrate metabolism,Immunology Inflammation,B Cell Receptor Signaling Pathway,Neuroscience,Calcium Signaling,Cardiovascular,Heart,Hypertrophy.

Images

Western blot analysis of lysates from Mouse brain, using Phospho-Camk2-T286 Rabbit pAb (AP0255) at 1:4000 dilution. Mouse brain cells were treated by CIP(20uL/400ul) at 37°C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.