Phospho-Smad1-S206 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-Smad1-S206 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0295
• Supplier Catalog Number: AP0295
• Alternative Catalog Number: ABB-AP0295-20UL,ABB-AP0295-1000UL,ABB-AP0295-500UL,ABB-AP0295-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: BSP1, JV41, BSP-1, JV4-1, MADH1, MADR1, Phospho-Smad1-S206

Product Description

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 52kDa
• NCBI: 4086
• UniProt: Q15797
• Purity: Affinity purification
• Sequence: PHSPT
• Target: SMAD1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Cancer,Signal Transduction,Cell Biology Developmental Biology,Growth factors,TGF-b-Smad Signaling Pathway,ESC Pluripotency and Differentiation,Immunology Inflammation,Stem Cells.

Images

Western blot analysis of lysates from C6 cells, using Phospho-Smad1-S206 Rabbit pAb (AP0295) at 1:1000 dilution. C6 cells were treated with UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.