Phospho-Casein Kinase 2 alpha (CSNK2A1)-T360/S362 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-Casein Kinase 2 alpha (CSNK2A1)-T360/S362 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0335
• Supplier Catalog Number: AP0335
• Alternative Catalog Number: ABB-AP0335-100UL, ABB-AP0335-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: CKII, Cka1, Cka2, CK2A1, OCNDS, Phospho-Casein Kinase 2 alpha (CSNK2A1)-T360/S362

Product Description

Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. Multiple transcript variants encoding different protein isoforms have been found for this gene.

Product Properties

• Molecular Weight: 45kDa
• NCBI: 1457
• UniProt: P68400
• Purity: Affinity purification
• Target: CSNK2A1

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse, ResearchArea: Epigenetics Nuclear Signaling,Epigenetic writers and erasers of core Histones,Signal Transduction,Kinase,Serine threonine kinases,Cell Biology Developmental Biology,Apoptosis,Cell Cycle,Cell Adhesion,Immunology Inflammation,NF-kB Signaling Pathway.

Images

Western blot analysis of lysates from 293F cells usingPhospho-Casein Kinase 2 alpha (CSNK2A1)-T360/S362 Rabbit pAb (AP0335) at1:400 dilution. 293F cells were treated with ERK2 (100ng/ml) at 37°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:60s.