Phospho-ESRalpha-S167 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-ESRalpha-S167 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0348
• Supplier Catalog Number: AP0348
• Alternative Catalog Number: ABB-AP0348-20UL, ABB-AP0348-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: ER, ESR, Era, ESRA, ESTRR, NR3A1, Phospho-ESRalpha-S167

Product Description

This gene encodes an estrogen receptor and ligand-activated transcription factor. The canonical protein contains an N-terminal ligand-independent transactivation domain, a central DNA binding domain, a hinge domain, and a C-terminal ligand-dependent transactivation domain. The protein localizes to the nucleus where it may form either a homodimer or a heterodimer with estrogen receptor 2. The protein encoded by this gene regulates the transcription of many estrogen-inducible genes that play a role in growth, metabolism, sexual development, gestation, and other reproductive functions and is expressed in many non-reproductive tissues. The receptor encoded by this gene plays a key role in breast cancer, endometrial cancer, and osteoporosis. This gene is reported to have dozens of transcript variants due to the use of alternate promoters and alternative splicing, however, the full-length nature of many of these variants remain uncertain.

Product Properties

• Molecular Weight: 66kDa
• NCBI: 2099
• UniProt: P03372
• Purity: Affinity purification
• Target: ESR1

Application Notes

• Application Dilute: WB,1:500 - 1:2000|IF/ICC,1:100 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Nuclear Receptor Signaling,Protein phosphorylation,Signal Transduction,MAPK-Erk Signaling Pathway.

Images

Western blot analysis of lysates from MCF7 cells using Phospho-ESRalpha-S167 Rabbit pAb (AP0348).
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.