Phospho-MARCKS-S162 Rabbit pAb, Unconjugated
Catalog Number:
ABB-AP0403
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| Article Name: | Phospho-MARCKS-S162 Rabbit pAb, Unconjugated |
| Biozol Catalog Number: | ABB-AP0403 |
| Supplier Catalog Number: | AP0403 |
| Alternative Catalog Number: | ABB-AP0403-1000UL,ABB-AP0403-100UL,ABB-AP0403-500UL,ABB-AP0403-20UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | MACS, 80K-L, PKCSL, PRKCSL, Phospho-MARCKS-S162 |
| The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. |
| Application Dilute: | WB,1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Signal Transduction,Kinase,Cell Biology Developmental Biology,Cytoskeleton,Endocrine Metabolism,Neuroscience,Calcium Signaling,Cardiovascular. |
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Western blot analysis of lysates from 293T cells, using Phospho-MARCKS-S162 Rabbit pAb (AP0403) at 1:400 dilution. 293T cells were treated with PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
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