Phospho-TP73-Y99 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-TP73-Y99 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0465
• Supplier Catalog Number: AP0465
• Alternative Catalog Number: ABB-AP0465-500UL,ABB-AP0465-1000UL,ABB-AP0465-20UL,ABB-AP0465-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: P73, CILD47, Phospho-TP73-Y99

Product Description

This gene encodes a member of the p53 family of transcription factors involved in cellular responses to stress and development. It maps to a region on chromosome 1p36 that is frequently deleted in neuroblastoma and other tumors, and thought to contain multiple tumor suppressor genes. The demonstration that this gene is monoallelically expressed (likely from the maternal allele), supports the notion that it is a candidate gene for neuroblastoma. Many transcript variants resulting from alternative splicing and/or use of alternate promoters have been found for this gene, but the biological validity and the full-length nature of some variants have not been determined.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 70kDa
• NCBI: 7161
• UniProt: O15350
• Purity: Affinity purification
• Target: TP73
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Cancer,Tumor suppressors,p53 pathway,ATM Signaling Pathway,Cell Biology Developmental Biology,Apoptosis.

Images

Western blot analysis of lysates from MCF7 cells, using Phospho-TP73-Y99 Rabbit pAb (AP0465) at 1:500 dilution. MCF7 cells were treated with pervanadate (1 mM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.