This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
WB,1:500 - 1:2000|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Immunoprecipitation analysis of 200 µg extracts of Jurkat cells, using 3 µg Phospho-ZAP70-Y319 pAb (AP0467). Western blot was performed from the immunoprecipitate using Phospho-ZAP70-Y319 pAb (AP0467) at a dilution of 1:1000. Jurkat cells were treated with Hydrogen Peroxide (2 mM) at 37°C for 2 minutes after serum-starvation overnight.
* VAT and and shipping costs not included. Errors and price changes excepted
