Phospho-PKC delta-Y311 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-PKC delta-Y311 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0496
• Supplier Catalog Number: AP0496
• Alternative Catalog Number: ABB-AP0496-20UL,ABB-AP0496-1000UL,ABB-AP0496-100UL,ABB-AP0496-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: MAY1, PKCD, ALPS3, CVID9, nPKC-delta, Phospho-PKC delta-Y311

Product Description

The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 78kDa
• NCBI: 5580
• UniProt: Q05655
• Purity: Affinity purification
• Sequence: VGIYQ
• Target: PRKCD
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,G protein signaling,G-Protein-Coupled Receptors Signaling to MAPK Erk,Kinase,Serine threonine kinases,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Mitochondrial Control of Apoptosis,Inhibition of Apoptosis,TGF-b-Smad Signaling Pathway,Immunology Inflammation,B Cell Receptor Signaling Pathway,Neuroscience.

Images

Western blot analysis of lysates from U937 and Raw264.7 cells, using Phospho-PKC delta-Y311 Rabbit pAb (AP0496) at 1:1000 dilution. U-937 cells were treated with PMA/TPA (200nM) for 30 minutes.RAW 264.7 cells were treated with PMA (200 nM) for 30 minutes .
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.