Phospho-AURKA-T288 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-AURKA-T288 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0523
• Supplier Catalog Number: AP0523
• Alternative Catalog Number: ABB-AP0523-20UL, ABB-AP0523-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: AIK, ARK1, AURA, BTAK, STK6, STK7, STK15, PPP1R47, Phospho-AURKA-T288

Product Description

The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.

Product Properties

• Molecular Weight: 46kDa
• NCBI: 6790
• UniProt: O14965
• Purity: Affinity purification
• Target: AURKA

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Protein phosphorylation,Cancer,Tumor biomarkers,Signal Transduction,Kinase,Serine threonine kinases,Cell Biology Developmental Biology,Cell Cycle,Centrosome,Cell Cycle Control-G2 M DNA Damage Checkpoint.

Images

Western blot analysis of various lysates, using Phospho-AURKA-T288 Rabbit pAb (AP0523) at 1:1000 dilution. HeLa were treated with Hydroxyurea (4mM) or treated with Nocodazole (50ng/mL) for 20 hours. HT-29 cells were treated with Hydroxyurea (4 mM) for 20 hours or treated with Nocodazole (100ng/mL) for 16 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.