Phospho-FLT3-Y589/591 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-FLT3-Y589/591 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0529
• Supplier Catalog Number: AP0529
• Alternative Catalog Number: ABB-AP0529-20UL, ABB-AP0529-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: FLK2, STK1, CD135, FLK-2, Phospho-FLT3-Y589/591

Product Description

This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

Product Properties

• Molecular Weight: 113kDa
• NCBI: 2322
• UniProt: P36888
• Purity: Affinity purification
• Target: FLT3

Application Notes

• Application Dilute: WB,1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Protein phosphorylation,Signal Transduction,Kinase,Tyrosine kinases,Immunology Inflammation,CDs,Stem Cells.

Images

Western blot analysis of lysates from A-431 cells, using Phospho-FLT3-Y589/591 Rabbit pAb (AP0529) at 1:500 dilution. A-431 cells were treated with pervanadate (1 mM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.