Phospho-MET-Y1234/1235 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-MET-Y1234/1235 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0533
• Supplier Catalog Number: AP0533
• Alternative Catalog Number: ABB-AP0533-100UL,ABB-AP0533-500UL,ABB-AP0533-20UL,ABB-AP0533-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: DA11, HGFR, AUTS9, RCCP2, c-Met, DFNB97, Phospho-MET-Y1234/1235

Product Description

This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 156kDa
• NCBI: 4233
• UniProt: P08581
• Purity: Affinity purification
• Sequence: KEYYSV
• Target: MET
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Protein phosphorylation,Cancer,Signal Transduction,Kinase,Tyrosine kinases,Cell Biology Developmental Biology,Growth factors,Stem Cells.

Images

Western blot analysis of lysates from HeLa cells, using Phospho-MET-Y1234/1235 Rabbit pAb (AP0533) at 1:1000 dilution. HeLa cells were treated with HGF (40 ng/ml) at 37°C for 10 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.