Phospho-PKC mu-S744/748 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-PKC mu-S744/748 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0534
• Supplier Catalog Number: AP0534
• Alternative Catalog Number: ABB-AP0534-100UL, ABB-AP0534-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: PKD, PKCM, CHDED, PRKCM, PKC-MU, Phospho-PKC mu-S744/748

Product Description

The protein encoded by this gene is a serine/threonine protein kinase involved in many cellular processes, including Golgi body membrane integrity and transport, cell migration and differentiation, MAPK8/JNK1 and Ras pathway signaling, MAPK1/3 (ERK1/2) pathway signaling, cell survival, and regulation of cell shape and adhesion.

Product Properties

• Molecular Weight: 102kDa
• NCBI: 5587
• UniProt: Q15139
• Purity: Affinity purification
• Target: PRKD1

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,G protein signaling,G-Protein-Coupled Receptors Signaling to MAPK Erk,Kinase,Serine threonine kinases,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Mitochondrial Control of Apoptosis,Inhibition of Apoptosis,Autophagy,Cytoskeleton,Actins,Endocrine Metabolism,Lipid Metabolism,Immunology Inflammation,B Cell Receptor Signaling Pathway.

Images

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PKC mu-S744/748 pAb (AP0534) at 1:1000 dilution or PRKD1 antibody (A16185). NIH/3T3 cells were treated with PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.