Phospho-IRS1-S636/639 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-IRS1-S636/639 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0551
• Supplier Catalog Number: AP0551
• Alternative Catalog Number: ABB-AP0551-20UL,ABB-AP0551-100UL,ABB-AP0551-1000UL,ABB-AP0551-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: HIRS-1, Phospho-IRS1-S636/639

Product Description

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 132kDa
• NCBI: 3667
• UniProt: P35568
• Purity: Affinity purification
• Sequence: MSPKSV
• Target: IRS1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Cancer,Signal Transduction,PI3K-Akt Signaling Pathway,mTOR Signaling Pathway,MAPK-Erk Signaling Pathway,MAPK-JNK Signaling Pathway,Cell Biology Developmental Biology,Growth factors,Endocrine Metabolism,Insulin Receptor Signaling Pathway,Endocrine and metabolic diseases,Diabetes,Obesity,Immunology Inflammation,Neuroscience, Cell Type Marker,Cardiovascular,Heart,Cardiovascular diseases,Heart disease.

Images

Western blot analysis of lysates from 293T cells, using Phospho-IRS1-S636/639 Rabbit pAb (AP0551) at 1:500 dilution. 293T cells were treated with IGF-1 (50 ng/ml) at 37°C for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.