Phospho-ALOX5-S271 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-ALOX5-S271 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0573
• Supplier Catalog Number: AP0573
• Alternative Catalog Number: ABB-AP0573-500UL,ABB-AP0573-100UL,ABB-AP0573-20UL,ABB-AP0573-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: 5-LO, 5LPG, LOG5, 5-LOX, Phospho-ALOX5-S271

Product Description

This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 78kDa
• NCBI: 240
• UniProt: P09917
• Purity: Affinity purification
• Sequence: QLSLE
• Target: ALOX5
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,Endocrine Metabolism,Lipid Metabolism,Cardiovascular,Heart,Cardiovascular diseases,Heart disease.

Images

Western blot analysis of lysates from HT-29 cells, using Phospho-ALOX5-S271 Rabbit pAb (AP0573) at 1:1000 dilution. HT-29 cells were treated by PMA/TPA (200nM).
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.