Phospho-beta-Catenin-S45 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-beta-Catenin-S45 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0580
• Supplier Catalog Number: AP0580
• Alternative Catalog Number: ABB-AP0580-20UL, ABB-AP0580-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: EVR7, CTNNB, MRD19, NEDSDV, armadillo, Phospho-beta-Catenin-S45

Product Description

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.

Product Properties

• Molecular Weight: 85kDa
• NCBI: 1499
• UniProt: P35222
• Purity: Affinity purification
• Target: CTNNB1

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Protein phosphorylation,Cancer,Invasion and Metastasis,Signal Transduction,ErbB-HER Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Cell Adhesion,Cadherins,Tight Junctions,Cytoskeleton,Microfilaments,Wnt -Catenin Signaling Pathway,ESC Pluripotency and Differentiation,Immunology Inflammation,Neuroscience,Stem Cells,Cardiovascular,Angiogenesis.

Images

Western blot analysis of lysates from HeLa cells, using Phospho-beta-Catenin-S45 Rabbit pAb (AP0580) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.