Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0588
• Supplier Catalog Number: AP0588
• Alternative Catalog Number: ABB-AP0588-20UL,ABB-AP0588-1000UL,ABB-AP0588-500UL,ABB-AP0588-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: MK2, MK-2, MAPKAP-K2, Phospho-MAPKAPK-2/MK2-T334

Product Description

This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 46kDa
• NCBI: 9261
• UniProt: P49137
• Purity: Affinity purification
• Sequence: PQTPL
• Target: MAPKAPK2
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation,Signal Transduction,G protein signaling,G-Protein-Coupled Receptors Signaling to MAPK Erk,Kinase,Serine threonine kinases,Cell Biology Developmental Biology,Cytoskeleton,Microfilaments,Microtubules,Endocrine Metabolism,Lipid Metabolism,Immunology Inflammation,Cardiovascular,Blood.

Images

Western blot analysis of various lysates using Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0588) at 1:1000 dilution. NIH/3T3 cells were treated with UV at room temperature for 15-30 minutes. C6 cells were treated with PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.