Phospho-BCR-Y177 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-BCR-Y177 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0606
• Supplier Catalog Number: AP0606
• Alternative Catalog Number: ABB-AP0606-100UL, ABB-AP0606-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: ALL, CML, PHL, BCR1, D22S11, D22S662, Phospho-BCR-Y177

Product Description

A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The unregulated tyrosine kinase activity of BCR-ABL1 contributes to the immortality of leukaemic cells. The BCR protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac and other kinases. Two transcript variants encoding different isoforms have been found for this gene.

Product Properties

• Molecular Weight: 143kDa
• NCBI: 613
• UniProt: P11274
• Purity: Affinity purification
• Target: BCR

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,G protein signaling,Small G proteins,Kinase,Serine threonine kinases,PI3K-Akt Signaling Pathway,Immunology Inflammation,B Cell Receptor Signaling Pathway,NF-kB Signaling Pathway.

Images

Western blot analysis of lysates from K562 cells, using Phospho-BCR-Y177 Rabbit pAb (AP0606) at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour .
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.