Phospho-CD115/CSF-1R-Y708 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-CD115/CSF-1R-Y708 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0609
• Supplier Catalog Number: AP0609
• Alternative Catalog Number: ABB-AP0609-20UL,ABB-AP0609-100UL,ABB-AP0609-1000UL,ABB-AP0609-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: FMS, CSFR, FIM2, GPSC, HDLS, C-FMS, CD115, HDLS1, CSF-1R, BANDDOS, M-CSF-R, Phospho-CD115/CSF-1R-Y708

Product Description

The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Alternative splicing results in multiple transcript variants. Expression of a splice variant from an LTR promoter has been found in Hodgkin lymphoma (HL), HL cell lines and anaplastic large cell lymphoma.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 108kDa
• NCBI: 1436
• UniProt: P07333
• Purity: Affinity purification
• Sequence: KKYVR
• Target: CSF1R
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Protein phosphorylation,Signal Transduction,Kinase,Tyrosine kinases,Immunology Inflammation,CDs,Cytokines,Cell Intrinsic Innate Immunity Signaling Pathway,Stem Cells,Cardiovascular,Angiogenesis.

Images

Western blot analysis of lysates from K-562 cells, using Phospho-CD115/CSF-1R-Y708 Rabbit pAb (AP0609) at 1:1000 dilution. K-562 cells were treated with G-CSF (25 ng/ml) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.