Phospho-Caveolin-1-Y14 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-Caveolin-1-Y14 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0742
• Supplier Catalog Number: AP0742
• Alternative Catalog Number: ABB-AP0742-100UL,ABB-AP0742-20UL,ABB-AP0742-500UL,ABB-AP0742-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: CGL3, PPH3, BSCL3, LCCNS, VIP21, MSTP085, Phospho-Caveolin-1-Y14

Product Description

The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 20kDa
• NCBI: 857
• UniProt: Q03135
• Purity: Affinity purification
• Sequence: HLYTV
• Target: CAV1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:1000 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse, ResearchArea: Protein phosphorylation,Cancer,Tumor biomarkers,Signal Transduction,ErbB-HER Signaling Pathway,Cell Biology Developmental Biology,Cell Adhesion,Endocrine Metabolism,Lipid Metabolism,Cholesterol Metabolism,Insulin Receptor Signaling Pathway,Cardiovascular,Hypoxia,Lipids.

Images

Western blot analysis of various lysates using Phospho-Caveolin-1-Y14 Rabbit pAb (AP0742) at 1:1000 dilution. Both HeLa cells and NIH/3T3 cells were treated with Hydrogen Peroxide (2 nM) at 37°C for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 120s.