Phospho-eIF4E-S209 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-eIF4E-S209 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0747
• Supplier Catalog Number: AP0747
• Alternative Catalog Number: ABB-AP0747-100UL,ABB-AP0747-20UL,ABB-AP0747-500UL,ABB-AP0747-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: CBP, EIF4F, AUTS19, EIF4E1, eIF-4E, EIF4EL1, Phospho-eIF4E-S209

Product Description

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5 end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 25kDa
• NCBI: 1977
• UniProt: P06730
• Purity: Affinity purification
• Sequence: SGSTT
• Target: EIF4E
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse, ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Signal Transduction,Endocrine Metabolism,Insulin Receptor Signaling Pathway,Immunology Inflammation,Cardiovascular,Angiogenesis.

Images

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-eIF4E-S209 Rabbit pAb (AP0747) at 1:500 dilution. NIH/3T3 cells were treated with 10% FBS at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.