Phospho-Glucocorticoid Receptor-S211 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-Glucocorticoid Receptor-S211 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0759
• Supplier Catalog Number: AP0759
• Alternative Catalog Number: ABB-AP0759-20UL, ABB-AP0759-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: GR, GCR, GRL, GCCR, GCRST, Phospho-Glucocorticoid Receptor-S211

Product Description

This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175).

Product Properties

• Molecular Weight: 86kDa
• NCBI: 2908
• UniProt: P04150
• Purity: Affinity purification
• Target: NR3C1

Application Notes

• Application Dilute: WB,1:1000 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Nuclear Receptor Signaling,Nuclear hormone receptors,Protein phosphorylation,Signal Transduction.

Images

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Glucocorticoid Receptor-S211 Rabbit pAb (AP0759) at 1:600 dilution. NIH/3T3 cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.