Phospho-ULK1-S555 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-ULK1-S555 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0760
• Supplier Catalog Number: AP0760
• Alternative Catalog Number: ABB-AP0760-100UL,ABB-AP0760-20UL,ABB-AP0760-1000UL,ABB-AP0760-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: ATG1, ATG1A, UNC51, hATG1, Unc51.1, Phospho-ULK1-S555

Product Description

Enables identical protein binding activity, protein serine/threonine kinase activity, and small GTPase binding activity. Involved in several processes, including autophagosome assembly, positive regulation by symbiont of host autophagy, and protein phosphorylation. Located in autophagosome, cytosol, and phagophore assembly site membrane. Is extrinsic component of autophagosome membrane, extrinsic component of omegasome membrane, and extrinsic component of phagophore assembly site membrane. Part of Atg1/ULK1 kinase complex.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 113 kDa
• NCBI: 8408
• UniProt: O75385
• Purity: Affinity purification
• Sequence: RLHSA
• Target: ULK1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:5000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Protein phosphorylation,Signal Transduction,Kinase,Serine threonine kinases,PI3K-Akt Signaling Pathway,Cell Biology Developmental Biology,Autophagy,Endocrine Metabolism,Mitochondrial metabolism,Neuroscience,Cardiovascular,Heart.

Images

Western blot analysis of lysates from 293T cells, using Phospho-ULK1-S555 Rabbit pAb (AP0760) at 1:1000 dilution. 293T cells were treated with Oligomycin (0. 5 uM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.